Genetics
Maintenance of C. elegans
Culturing worms, Bacteria for Worm Food, Seeding Plates, Cleaning Worms Strains without bleach, Cleaning worms by floating on sucrose, Freezing Worms, ...
- Culturing Worms (Michael Koelle Lab)
- Bacteria for Worm Food and Seeding Plates (Loer Lab)
- Cleaning Worm Strains (Michael Koelle Lab)
- Cleaning worms by floating on sucrose (Ambros Lab)
- Freezing Worms (Michael Koelle)
Liquid culture of C. elegans
Growing worms in liquid, Liquid culture of worms, Liquid culture of worms with fermentor, ...
- Growing worms in liquid (Herman Lab)
- Liquid culture of worms (Michael Koelle)
- LIQUID CULTURE OF WORMS WITH FERMENTOR (Ambros Lab)
Mating of C. elegans and Generating Males
- Generating males by heat shock (Michael Koelle)
Generating males by heat shock, ...
Making C. elegans Dauers
- Dauer Pheromone Prep (Cori Bargmann and Brenda Reinhart 1992)
Dauer Pheromone Prep, ...
Primary Cell Cuture
- Primary culture of C. elegans embryonic neurons (Laird Bloom PhD Thesis, MIT)
Primary culture of C. elegans embryonic neurons, ...
RNAi (RNA Interference)
- dsRNA prep protocol (Goldstein lab)
Preparation of dsRNA for microinjection, ...
Mutagenesis and Genetic Screens
EMS mutagenesis, C. elegans Gene Knockout Protocol, F2 Mutant Screen - non "clonal", ...
- EMS mutagenesis (Michael Koelle)
- EMS mutagenesis (Herman Lab)
- F2 Mutant Screen - non "clonal" (Michael Koelle)
- C. elegans Gene Knockout Protocol (Michael Koelle)
Integrating extrachromasomal arrays into the C. elegans chromosomes
Integrating extrachromasomal arrays into the C. elegans chromosomes, MMS integration, ...
- Integrating extrachromasomal arrays into the C. elegans chromosomes (Michael Koelle)
- MMS integration (Herman Lab)
Microinjection of Worms
Microinjecting worms, ...
- Microinjecting worms (Michael Koelle)
Gonad Disections
Gonad Dissection, ...
- Gonad Dissections (Schedl Lab, Washington University)
More Worm Protocols
Egg laying behavior in response to food, Lethal phase determination, ...
- Egg laying behavior in response to food (Michael Koelle Lab)
- Lethal phase determination (Michael Koelle Lab)
Nucleic Acids
- Worm genomic Southern blots (Michael Koelle)
- Single Worm PCR (Herman Lab)
- C. elegans RNA prep (Michael Koelle)
- dsRNA prep protocol (Goldstein lab)
References
- C. elegans Spliced leader (SL) RNA Sequences
- Worm Codon Preferences (Ambros Lab)