| PubMed I Cell Nature Science Scientific American I HHMI Nobelprize I Weather |
40% Methanol
10% Acetic acid
5% Glycerol
45% H20
I recommend this protocol for drying thicker polyacrylamide gels. It's good for both denaturing and non-denaturing gels. You do not need to treat your gels with gel-soak if your gels are thin. You can directly dry thin gels on gel dryer.
Followings are my experience with gels, yours could vary depends on gel thickness etc:
You do not need to treat non-denaturing gels that are 11% or thinner with Gel-Soak.
You do not need to treat denaturing gels that are 6% or thinner with Gel-Soak. However, I have never tried drying a denaturing gel that is higher percentage than 6%. My guess you may go up to 7% or 8% without Gel-Soak treatment.
After electrophoresis is done, soak the gel in gel soak for 30 minutes to 1 hour. Gel soak gets hot if there is unincorporated nucleotide in your gel. I recommend a second rinse step after pulling off the first gel soak. Dry the gel by a gel dryer. It may take overnight to completely dry the gel. Good luck!