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Cell Lysis Buffers or Protein Lysis Buffers

NP-40 Lysis buffer:

150 mM   NaCl
1.0% NP-40
50 mM Tris-HCl pH 8.0  

High Salt Lysis Buffer:

500 mM   NaCl
1.0% NP-40
50 mM Tris-HCl pH 8.0  

Low Salt Lysis Buffer:

1.0% NP-40
50 mM   Tris-HCl pH 8.0  

RIPA Buffer:

150 mM   NaCl
1% NP-40
0.5% NaDeoxycholate  
0.1% SDS
50 mM Tris-HCl pH 8.0

Good luck with your antibody work!