| Reagent |
Target/Notes |
Stock |
Stock Preparation |
Final Concentration |
| Aprotinin |
protease |
Sigma |
n/a |
1/100 |
| Benzamidine |
competitive inhibitor of trypsin, trypsin-like enzymes, and serine proteases |
250 mM |
|
1 mM |
| Diisopropyl fluorophosphate |
protease |
100mM |
100mM in anhydrous 2-propanol. Powerful Neurotoxin |
1mM |
| Iodoacetamide |
protease/phosphatase |
500mM |
93 mg/ml |
5mM |
| Leupeptin |
protease |
400 mM |
200 mg/ml |
4 mM |
| Na2EDTA (sodium EDTA) |
protease |
500 mM pH 8.0 |
n/a chelates vanadate |
5 mM |
| Na2EGTA (sodium EGTA) |
protease |
500 mM pH8.0 |
n/a no vanadate chelation |
5 mM |
| Na3VO4 (sodium vanadate) |
phosphatase |
20-40mM |
7.4 mg/ml |
0.2-0.4mM |
| Na3VO4 (peroxide treated sodium vanadate) |
phosphatase |
20mM |
20mM Na3VO4, 15mM H2O2, 5min RT, then 200µg/ml catalase |
0.2mM |
| NaF (sodium fluoride) |
phosphatase |
1 M |
42 mg/ml |
10 mM |
| Na2MoO4 (sodium molybdate) |
phosphatase |
1 M |
242 mg/ml |
10 mM |
| Na4P2O7 (sodium pyrophosphate) |
phosphatase |
1 M |
446 mg/ml |
10 mM |
| Pepstatin A |
protease |
100 µM |
0.07 mg/ml in anhydrous 2-propanol |
1 µM |
| PAO (phenylarsine oxide) |
phosphatase |
100 mM |
16.8 mg/ml in DMSO, serious contact hazard |
50µM |
| PMSF (phenyl methyl sulfonyl) |
protease |
100 mM |
17.4 mg/ml in ethanol or in anhydrous 2-propanol |
0.5-2 mM |
| Trypsin inhibitor |
protease |
1 mg/ml |
1 mg/ml |
10 µg/ml |