Coomassie Blue Staining SDS PAGE Gels
Method
- Immerse the gel in the staining solution.
- Microwave for 30 seconds.
- Place the gel on a rotating platform and slowly shake until bands become visible. Takes about half an hour (microwave is used to speed up the staining process).
- Dump off the staining solution and save for future use.
- Rinse the gel several times with ultrapure water.
- Dump off ultrapure water
- Immerse the gel in the destaining solution.
- Microwave for 30 seconds.
- Fold and place a single sheet of paper towel or several sheets of Kimwipes™ at the corner of the container (paper has proteins and binds to the stain, speeding up the destaining process).
- Place the gel back on a rotating platform and shake until bands become visible. This takes about half an hour. Alternatively, you can leave it overnight.
- Destain further by replacing the paper towel, if it is necessary.
Reagents Required
Staining Solution 1 liter:
- 500 ml Methanol
- 400 ml Ultrapure water
- 100 ml Glacial Acetic Acid
- 2.5 g Coomassie Brilliant Blue R-250
- Filter solution through Whatman No. 1 filter to remove impurities.
Destaining Solution 1 liter:
- 785 ml ultrapure water
- 165 ml Ethanol
- 50 ml Glacial Acetic Acid