1X SDS Sample Buffer consist of:
63 mM Tris HCl
0.0025% Bromophenol Blue
Reference for Laemmli Buffer:
The following recipe is for 2X SDS Sample Buffer:
|0.5 M Tris-HCl, pH 6.8||2.5 ml|
|10% (w/v) SDS||4 ml|
|0.1% (w/v) Bromophenol Blue||0.5 ml|
Bring the volume to 10 ml with ultra pure water.
Add Beta-Mercaptoethanol (2-Mercaptoethanol) at a final concentration of 2.5 to 5.0%. Beta-Mercaptoethanol is necessary to reduce intra or intermolecular disulfide bonds.
Denature samples by heating at 85 C for 2-5 min.
Good luck with your protein electrophoresis experiments!