| PubMed I Cell Nature Science Scientific American I HHMI Nobelprize I Weather |
Source-1:
The table below is directly taken from Finnzymes website.
| Buffer | Application Example | Activity (%) |
|---|---|---|
| 20 mM Tris-HCl, pH 8.0 | Reference | 100 |
| 10 mM Tris-HCl, 1 mM EDTA, 0.5 % SDS, pH 8.0 | Bacterial genomic DNA isolation | 108 |
| 10 mM Tris-HCl, 100 mM NaCl, 25 mM EDTA, 1 % SDS, pH 8.0 | Genomic DNA isolation from mammalian tissues | 171 |
| 100 mM Tris-HCl, 100 mM EDTA, 250 mM NaCl, 1 % Sarkosyl, pH 8.0 | Plant tissue genomic DNA isolation | 118 |
| 10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 5 mM EDTA, 0.5 % SDS, pH 7.9 | Inactivation of Calf Intestinal Alkaline Phosphatase | 104 |
| 50 mM Tris-HCl, 1 mM CaCl2, 3 mM DTT, 2 M Urea, pH 8.0 | Denaturation of proteins | 66 |
| 10 mM Tris-HCl, 1.5 mM MgCl2, 50 mM KCl, 0.1 % Triton X-100, pH 8.8 | Optimized DyNAzyme Buffer | 158 |
| 50 mM Tris-HCl, 1.5 mM MgCl2, 15 mM (NH4)2SO4, 0.1 % Triton X-100, pH 9.0 | Optimized DyNAzyme EXT Buffer | 168 |
Source-2:
The following information is directly taken from NEB website.
Buffer: 30 mM Tris HCl, pH 8.0
Application: Reference
Relative activity (approx.): 100%
Buffer: 50mM Tris-HCl, pH 8.0, 1mM CaCl2,3mM DTT, 2.0M Urea
Application: Denaturation of proteins
Relative activity (approx.): 70%
Buffer: 100mM Tris-HCl, pH 8.0, 100mM EDTA, 250mM NaCl, 1% Sarkosyl
Application: Plant tissue DNA isolation
Relative activity (approx.): 120%
Buffer: 10mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5% SDS
Application: Bacterial DNA isolation
Relative activity (approx.): 100%
Buffer: 10mM Tris HCl, pH 8.0, 50mM NaCl, 5mM EDTA, 1mM DTT, 0.5% SDS 50mM Tris-HCl, pH 8.0
Application: Denaturation of CIP
Relative activity (approx.): 100%
Buffer: 50mM EDTA, 5% Tween 20, 0.5% Triton-X 100, 800mM GuHCl
Application:
Relative activity (approx.): 300%