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TBE Buffer Recipe (Tris-Borate-EDTA)

10X TBE Buffer Preparation:

Mix following components to make 1 liter 10X TBE.



Component Amount 10X Stock Concentration Final 1X Concentration
Tris Base 108 g 890 mM 89 mM
Boric Acid 55 g 890 mM 89 mM
EDTA (pH 8.0) 40 ml 20 mM 2 mM

5X TBE Buffer Preparation:

Mix following components to make 1 liter 5X TBE.



Component Amount 5X Stock Concentration Final 1X Concentration
Tris Base 54 g 445 mM 89 mM
Boric Acid 27.5 g 445 MM 89 mM
EDTA (pH 8.0) 20 ml 10 mM 2 mM

Note: 1. Filtering by 0.22 micron filter delay precipitation. 10x TBE is more likely to precipitate. pH of stock solution will be around pH 8.3 (do not adjust pH!)

Good luck with your experiments!