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Following SSC buffer recipe is for total volume of 1 liter:
| Component | Amount | 20X Stock Concentration | Final 1X Concentration |
|---|---|---|---|
| NaCl | 175.3 g | 3 M | 150 mM |
| Na3CitrateX2H2O | 88.2 g | 300 mM | 15 mM |
Dissolve 175.3 g of NaCl and 88.2 g of Sodium Citrate in 800 ml water.
Adjust the pH to 7.0 with a few drops of 14 N solution of HCl.
Adjust the volume to 1 liter with ultrapure water.
Dispense into aliquots. Sterilize by autoclaving.
For 20X SSPE recipe, use the following link: 20X SSPE
Good luck with your DNA and RNA hybridization experiments!