| PubMed I Cell Nature Science Scientific American I HHMI Nobelprize I Weather |
| Component | Amount | Stock 20X concentration | Final 1X concentration |
|---|---|---|---|
| NaCl | 175.3g | 3 M | 150 mM |
| NaH2PO4xH2O | 27.6g | 200 mM | 10 mM |
| Na2EDTA | 7.4g | 200 mM | 10 mM |
Dissolve the reagents in 800 ml ultrapure water.
Adjust pH to 7.4 with 10 M NaOH.
Bring volume to 1 liter with ultrapure water.
Sterilize by autoclaving.
Good luck with your DNA and RNA hybridization experiments!