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Preparation of 50x TAE Buffer (Tris-Acetate-EDTA)

TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. To prepare 50x TAE Buffer, mix following reagents in a total volume of 1 liter ultrapure water:



242 g Tris Base
57.1 ml   Acetic Acid
100 ml 0.5 M EDTA (pH 8.0)

Good luck with your experiments!