free online protocols for biomedical research
PubMed  I  Cell    Nature   Science   Scientific American  I  HHMI    Nobelprize  I  Weather    
Home > Reagent And Buffer Recipes

Preparation of 50x TAE Buffer (Tris-Acetate-EDTA)

TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. To prepare 50x TAE Buffer, mix following reagents in a total volume of 1 liter ultrapure water:

242 g Tris Base
57.1 ml   Acetic Acid
100 ml 0.5 M EDTA (pH 8.0)

Good luck with your experiments!