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Home > Reagent And Buffer Recipes

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Recipes for the Preparation of Common Laboratory Reagents and Buffers

Commonly used Buffers and Solutions are listed alphabetically. You can use browser's search function to find protocols on this page. Good luck with your experiments!

A

B

C

Church's Buffer

Crush and Soak Buffer

D

Denhardt's (50X)

DNA loading dye (10X and 6X)

G

Glycine (2M)

H

HEPES Buffer (1M)

L

LB (L-Broth or Luria Bertani) Medium

Lysis Buffer, NP-40

Lysis Buffer, High Salt

Lysis Buffer, Low Salt

Lysis Buffer, RIPA Buffer

M

MOPS Running Buffer (10X)

N

NaCl (5 M)

O

Oligo Annealing Buffer

P

PBS Buffer (Phosphate Buffered Saline) (10X)

Proteinase K

S

SDS Sample Buffer

SOB Medium

SSC (20X)

SSPE (20X)

T

TAE Buffer (50X)

TBE Buffer (10X)

TBS Buffer (10X), Tris-Buffered Saline

TBST Buffer

TE (Tris-EDTA) Buffer (10X)

Tris-Glycine DNA Electrophoresis Buffer (5X)

Tris Buffer pH 7.5 (1 M)

Tris-HEPES-SDS Running Buffer (10X)

X

X-gal Stock Solution

Y

Yeast Media: YPD (YEPD)

Yeast Media: YPDA

Yeast Media: Synthetic