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10X PBS Buffer Recipe

1X PBS (Phosphate Buffered Saline) Buffer consists of:

To make 10X PBS Buffer, dissolve the following reagents in 800 ml ultrapure water.

NaCl 80 g
KCl 2 g
Na2HPO4 14.4 g
KH2PO4 2.72 g

Adjust pH of PBS Buffer Solution to 7.4 with HCl.
Bring volume to 1 liter, autoclave or sterilize by filtration.

Good luck with your experiments!