10X TBS: 500 mM Tris.HCl, pH 7.4 and 1500 mM NaCl.
1X TBS: 50 mM Tris.HCl, pH 7.4 and 150 mM NaCl.
To prepare 1000 ml of 10X TBS stock solution, mix the following reagents:
Note: Tris concentration in 1X TBS varies from 20 mM to 100 mM from recipe to recipe. 50 mM is a commonly used concentration.