free online protocols for biomedical research
PubMed  I  Cell    Nature   Science   Scientific American  I  HHMI    Nobelprize  I  Weather    
Home > Reagent And Buffer Recipes

1 M Tris Buffer Recipe (pH 7.5)

  1. Dissolve 121.1 g Tris in 800 ml of ulptrapure water.
  2. Add 65 ml concentrated HCl (This should bring pH to 7.5).
  3. Wait until solution reaches to room temperature, then measure the pH.
  4. Sterilize by heat.
Tris = Tris[hydroxymethyl]aminomethane = (HOCH2)3CNH2