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10X TE Buffer Recipe

This buffer consists of 100 mM Tris-HCl (pH 7.5) and 10 mM EDTA (pH 8.0). Working concentration is 1X or lower.

Make it from 1 M stock of Tris-HCl (pH 7.5) and 500 mM stock of EDTA (pH 8.0).
To make 1 liter 10X TE (Tris-EDTA) Buffer, mix following:

• 100 ml 1 M Tris-HCl pH 7.5
• 20 ml 500 mM EDTA pH 8.0
• 880 ml ultrapure water

Good luck with your experiments!