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Tris-HEPES-SDS Running Buffer (10X)

Recipe for 10X Tris-HEPES-SDS Buffer:

• Tris Base  121 g
• HEPES     238 g
• SDS           10 g
• Add ultrapure water to 1,000 ml

1X Tris-HEPES-SDS Running Buffer consists of:

• 100 mM Tris
• 100 mM HEPES
• 3 mM SDS

1X Tris-HEPES-SDS Running Buffer is used to run Precise Protein Gels (Pierce), and compatible with Tris-Glycine SDS sample loading buffer.