DNA Migration in Agarose and Polyacrylamide Gels

DNA Migration in Agarose and Polyacrylamide Gels

Recommended Gel Percentages for Separation of Linear DNA

Agarose gel %	Range of separation (bp)
0.5	1,000-30,000
0.7	800-12,000
1.0	500-10,000
1.2	400-7,000
1.4	200-4,000
2.0	50-2,000

Polyacrylamide gel %	Range of separation (bp)
3.5	100-1,000
5.0	80-500
8.0	60-400
12.0	40-200
20.0	5-100