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Tag: Medium

bioprotocols

DNA sequencing with chain-terminating inhibitors

Posted on June 27, 2021April 29, 2021 by Oscar

DNA sequencing with chain-terminating inhibitors A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors… Continue reading

bioprotocols

Cluster analysis and display of genome-wide expression patterns

Posted on May 21, 2021April 27, 2021 by Oscar

Cluster analysis and display of genome-wide expression patterns A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression. The output is displayed graphically, conveying the clustering and the underlying expression data simultaneously in a form intuitive… Continue reading

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