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Month: May 2021

bioprotocols

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome

Posted on May 25, 2021April 29, 2021 by Oscar

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome Bowtie is an ultrafast, memory-efficient alignment program for aligning short DNA sequence reads to large genomes. For the human genome, Burrows-Wheeler indexing allows Bowtie to align more than 25 million reads per CPU hour with a memory footprint of approximately 1.3 gigabytes. Bowtie extends previous Burrows-Wheeler techniques… Continue reading

bioprotocols

Cluster analysis and display of genome-wide expression patterns

Posted on May 21, 2021April 27, 2021 by Oscar

Cluster analysis and display of genome-wide expression patterns A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression. The output is displayed graphically, conveying the clustering and the underlying expression data simultaneously in a form intuitive… Continue reading

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