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Category: pcr

bioprotocols

DnaSP v5: a software for comprehensive analysis of DNA polymorphism data

Posted on June 27, 2021April 29, 2021 by Oscar

DnaSP v5: a software for comprehensive analysis of DNA polymorphism data DnaSP is a software package for a comprehensive analysis of DNA polymorphism data. Version 5 implements a number of new features and analytical methods allowing extensive DNA polymorphism analyses on large datasets. Among other features, the newly implemented methods allow for: (i) analyses on multiple data files; (ii) haplotype phasing;… Continue reading

bioprotocols

DNA sequencing with chain-terminating inhibitors

Posted on June 27, 2021April 29, 2021 by Oscar

DNA sequencing with chain-terminating inhibitors A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors… Continue reading

bioprotocols

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome

Posted on May 25, 2021April 29, 2021 by Oscar

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome Bowtie is an ultrafast, memory-efficient alignment program for aligning short DNA sequence reads to large genomes. For the human genome, Burrows-Wheeler indexing allows Bowtie to align more than 25 million reads per CPU hour with a memory footprint of approximately 1.3 gigabytes. Bowtie extends previous Burrows-Wheeler techniques… Continue reading

bioprotocols

Cluster analysis and display of genome-wide expression patterns

Posted on May 21, 2021April 27, 2021 by Oscar

Cluster analysis and display of genome-wide expression patterns A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression. The output is displayed graphically, conveying the clustering and the underlying expression data simultaneously in a form intuitive… Continue reading

Biosynthesis of g Hardware Design and Fault-Tolerant Synthesis for Digital Acoustofluidic Biochipsold nanoparticles by foliar broths: roles of biocompounds and other attributes of the extracts.

Posted on April 29, 2021April 29, 2021 by Oscar

Biosynthesis of gold nanoparticles by foliar broths: roles of biocompounds and other attributes of the extracts. Biosynthesis of nanoparticles has arisen as a promising alternative to conventional synthetic methodologies owing to its eco-friendly advantages, and the involved bioprotocol still needs further clarification. This research, for the first time from the standpoint of statistics, confirmed an electrostatic force… Continue reading

bioprotocols

Chromatin modifications and their function

Posted on April 27, 2021April 27, 2021 by Oscar

Chromatin modifications and their function The surface of nucleosomes is studded with a multiplicity of modifications. At least eight different classes have been characterized to date and many different sites have been identified for each class. Operationally, modifications function either by disrupting chromatin contacts or by affecting the recruitment of nonhistone proteins to chromatin. Their… Continue reading

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    • Advancements in Glycan Biosynthesis: Transforming the Advancement of Biotechnological Production and Therapeutics
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    • Innovations in Glycosylation Pathways: Defining the Future of Biomanufacturing Processes and Pharmaceutical Applications
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