Bioprotocols – Free online protocols for Biomedical Research
DNA extraction protocol, MSDS, Material Safety Data Sheet
Specifications Research area: Oxidative stress Assay Category: DNA / RNA Damage and Repair Assays Sample type: cultured cells, fresh and frozen tissues, paraffin-embedded tissues, body fluid samples, etc. Species reactivity: any species such as mammals, plants, fungi, bacteria, and viruses Detection method: colorimetric Product description Easy-to-follow steps for convenience and speed High sensitivity (see Fig.… Continue reading
Specification Article No: ABD-22900 Country Availability: SE, FI, DK, NO, IS, EE, LV, LT, RU Description Cell Meter ™ Fluorimetric Intracellular Total ROS Activity Assay Kit Provider: AAT Bioquest Product Type: Assay and detection Shipping information: RT Size: 200 tests Stability: 6 months Storage: f / l Technical Specifications Reactive Oxygen Species (ROS) are natural… Continue reading
Specification Application: IF, FC Article No: ABD-36316 Country Availability: SE, FI, DK, NO, IS, EE, LV, LT, RU Description Screen Quest ™ Fluo-8 Calcium No Wash Assay Kit Provider: AAT Bioquest Alias Names: Calcium indicator from Product Type: Assay and detection Shipping information: RT Size: Plate 100 Stability: 6 months Storage: f / l Technical… Continue reading
Product description The EpiQuik ™ In Situ DNA Damage Assay Kit is a practical set of tools that enables the experimenter to detect DNA damage or apoptosis by measuring H2AXSer139 phosphorylation in situ. The kit is ready to use and provides all the essential components necessary to specifically measure DNA damage in situ by detecting… Continue reading
Product details Applications: Flow cytometry (flow) Proven dilution: 0.25 µg / test Product specifications Species reactivity: Mouse Published species: Mouse Host / isotype: Rat / IgG2a, kappa Recommended isotype control Rat IgG2a kappa isotype control (eBR2a), eFluor 660, eBioscience Class: Monoclonal Kind: Antibody Clone: BE-4 Conjugate: eFluor® 660 See additional formats Excitation / emission profile:… Continue reading
A call among readers has raised many questions about the corona vaccine. In a lecture for Utrecht University and Tivoli, professor of vaccinology Cécile van Els answers many of these questions. Read here how it is. 1. I have already had corona. Does a vaccine make sense? Cécile van Els: ‘People react very differently after infection, the variation… Continue reading
DnaSP v5: a software for comprehensive analysis of DNA polymorphism data DnaSP is a software package for a comprehensive analysis of DNA polymorphism data. Version 5 implements a number of new features and analytical methods allowing extensive DNA polymorphism analyses on large datasets. Among other features, the newly implemented methods allow for: (i) analyses on multiple data files; (ii) haplotype phasing;… Continue reading
DNA sequencing with chain-terminating inhibitors A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors… Continue reading
Mfold web server for nucleic acid folding and hybridization prediction The abbreviated name, ‘mfold web server’, describes a number of closely related software applications available on the World Wide Web (WWW) for the prediction of the secondary structure of single stranded nucleic acids. The objective of this web server is to provide easy access to… Continue reading
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome Bowtie is an ultrafast, memory-efficient alignment program for aligning short DNA sequence reads to large genomes. For the human genome, Burrows-Wheeler indexing allows Bowtie to align more than 25 million reads per CPU hour with a memory footprint of approximately 1.3 gigabytes. Bowtie extends previous Burrows-Wheeler techniques… Continue reading