The fact is that siRNAs—the double-stranded effectors of RNA interference (RNAi)—can “be developed to silence any gene; can be developed incredibly fast; can have picomolar efficacy; [and] can have an effect that lasts for weeks.” In the lab, they have become invaluable tools, enabling the easy genetic knockdown of any sequence. The problem with migrating… Continue reading
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LB Medium and Agar Plate Recipe
Protocol for LB liquid medium: For 1 liter, dissolve 10 g Tryptone, 5 g yeast extract, and 10 g NaCl in 950 ml deionized water. Adjust the pH of the solution to 7.0 with NaOH and bring the volume up to 1 liter.It may not be necessary to adjust pH every time, since pH of LB… Continue reading
Material safety data
Use this calculator to prepare your solutions from solid reagents. Volume: L Molarity: M Molecular Weight: gram/mol Hit the submit button to calculate the weight of the reagent needed for the given volume to obtain desired molarity. Your employees should not have to guess whether or not they’re pulling up the correct SDS for… Continue reading
Molarity Calculator II
Use this calculator to adjust the concentration of a substance in a present solution to a higher concentration by adding a minimum volume of solution from a concentrated stock. Concentration of present solution: mM Volume of present solution: ml Stock Concentration: mM Desired Final Concentration: mM Hit the submit button to calculate the volume of… Continue reading