Bioprotocols – Free online protocols for Biomedical Research
DNA extraction protocol, MSDS, Material Safety Data Sheet
Mfold web server for nucleic acid folding and hybridization prediction The abbreviated name, ‘mfold web server’, describes a number of closely related software applications available on the World Wide Web (WWW) for the prediction of the secondary structure of single stranded nucleic acids. The objective of this web server is to provide easy access to… Continue reading
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome Bowtie is an ultrafast, memory-efficient alignment program for aligning short DNA sequence reads to large genomes. For the human genome, Burrows-Wheeler indexing allows Bowtie to align more than 25 million reads per CPU hour with a memory footprint of approximately 1.3 gigabytes. Bowtie extends previous Burrows-Wheeler techniques… Continue reading
Cluster analysis and display of genome-wide expression patterns A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression. The output is displayed graphically, conveying the clustering and the underlying expression data simultaneously in a form intuitive… Continue reading
Biosynthesis of gold nanoparticles by foliar broths: roles of biocompounds and other attributes of the extracts. Biosynthesis of nanoparticles has arisen as a promising alternative to conventional synthetic methodologies owing to its eco-friendly advantages, and the involved bioprotocol still needs further clarification. This research, for the first time from the standpoint of statistics, confirmed an electrostatic force… Continue reading