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Category: cDNA

bioprotocols

DNA sequencing with chain-terminating inhibitors

Posted on June 27, 2021April 29, 2021 by Oscar

DNA sequencing with chain-terminating inhibitors A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors… Continue reading

bioprotocols

Mfold web server for nucleic acid folding and hybridization prediction

Posted on June 15, 2021April 29, 2021 by Oscar

Mfold web server for nucleic acid folding and hybridization prediction The abbreviated name, ‘mfold web server’, describes a number of closely related software applications available on the World Wide Web (WWW) for the prediction of the secondary structure of single stranded nucleic acids. The objective of this web server is to provide easy access to… Continue reading

bioprotocols

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome

Posted on May 25, 2021April 29, 2021 by Oscar

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome Bowtie is an ultrafast, memory-efficient alignment program for aligning short DNA sequence reads to large genomes. For the human genome, Burrows-Wheeler indexing allows Bowtie to align more than 25 million reads per CPU hour with a memory footprint of approximately 1.3 gigabytes. Bowtie extends previous Burrows-Wheeler techniques… Continue reading

Biosynthesis of g Hardware Design and Fault-Tolerant Synthesis for Digital Acoustofluidic Biochipsold nanoparticles by foliar broths: roles of biocompounds and other attributes of the extracts.

Posted on April 29, 2021April 29, 2021 by Oscar

Biosynthesis of gold nanoparticles by foliar broths: roles of biocompounds and other attributes of the extracts. Biosynthesis of nanoparticles has arisen as a promising alternative to conventional synthetic methodologies owing to its eco-friendly advantages, and the involved bioprotocol still needs further clarification. This research, for the first time from the standpoint of statistics, confirmed an electrostatic force… Continue reading

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