Bioprotocols – Free online protocols for Biomedical Research
DNA extraction protocol, MSDS, Material Safety Data Sheet
Cluster analysis and display of genome-wide expression patterns A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression. The output is displayed graphically, conveying the clustering and the underlying expression data simultaneously in a form intuitive… Continue reading
Biosynthesis of gold nanoparticles by foliar broths: roles of biocompounds and other attributes of the extracts. Biosynthesis of nanoparticles has arisen as a promising alternative to conventional synthetic methodologies owing to its eco-friendly advantages, and the involved bioprotocol still needs further clarification. This research, for the first time from the standpoint of statistics, confirmed an electrostatic force… Continue reading
Chromatin modifications and their function The surface of nucleosomes is studded with a multiplicity of modifications. At least eight different classes have been characterized to date and many different sites have been identified for each class. Operationally, modifications function either by disrupting chromatin contacts or by affecting the recruitment of nonhistone proteins to chromatin. Their… Continue reading
The fact is that siRNAs—the double-stranded effectors of RNA interference (RNAi)—can “be developed to silence any gene; can be developed incredibly fast; can have picomolar efficacy; [and] can have an effect that lasts for weeks.” In the lab, they have become invaluable tools, enabling the easy genetic knockdown of any sequence. The problem with migrating… Continue reading
Protocol for LB liquid medium: For 1 liter, dissolve 10 g Tryptone, 5 g yeast extract, and 10 g NaCl in 950 ml deionized water. Adjust the pH of the solution to 7.0 with NaOH and bring the volume up to 1 liter.It may not be necessary to adjust pH every time, since pH of LB… Continue reading
Use this calculator to prepare your solutions from solid reagents. Volume: L Molarity: M Molecular Weight: gram/mol Hit the submit button to calculate the weight of the reagent needed for the given volume to obtain desired molarity. Your employees should not have to guess whether or not they’re pulling up the correct SDS for… Continue reading
Use this calculator to adjust the concentration of a substance in a present solution to a higher concentration by adding a minimum volume of solution from a concentrated stock. Concentration of present solution: mM Volume of present solution: ml Stock Concentration: mM Desired Final Concentration: mM Hit the submit button to calculate the volume of… Continue reading