DNA extraction protocol, MSDS, Material Safety Data Sheet
Product details Applications: Flow cytometry (flow) Proven dilution: 0.25 µg / test Product specifications Species reactivity: Mouse Published species: Mouse Host / isotype: Rat / IgG2a, kappa Recommended isotype control Rat IgG2a kappa isotype control (eBR2a), eFluor 660, eBioscience Class: Monoclonal Kind: Antibody Clone: BE-4 Conjugate: eFluor® 660 See additional formats Excitation / emission profile:… Continue reading
A call among readers has raised many questions about the corona vaccine. In a lecture for Utrecht University and Tivoli, professor of vaccinology Cécile van Els answers many of these questions. Read here how it is. 1. I have already had corona. Does a vaccine make sense? Cécile van Els: ‘People react very differently after infection, the variation… Continue reading
DnaSP v5: a software for comprehensive analysis of DNA polymorphism data DnaSP is a software package for a comprehensive analysis of DNA polymorphism data. Version 5 implements a number of new features and analytical methods allowing extensive DNA polymorphism analyses on large datasets. Among other features, the newly implemented methods allow for: (i) analyses on multiple data files; (ii) haplotype phasing;… Continue reading
DNA sequencing with chain-terminating inhibitors A new method for determining nucleotide sequences in DNA is described. It is similar to the “plus and minus” method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2′,3′-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors… Continue reading
Mfold web server for nucleic acid folding and hybridization prediction The abbreviated name, ‘mfold web server’, describes a number of closely related software applications available on the World Wide Web (WWW) for the prediction of the secondary structure of single stranded nucleic acids. The objective of this web server is to provide easy access to… Continue reading
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome Bowtie is an ultrafast, memory-efficient alignment program for aligning short DNA sequence reads to large genomes. For the human genome, Burrows-Wheeler indexing allows Bowtie to align more than 25 million reads per CPU hour with a memory footprint of approximately 1.3 gigabytes. Bowtie extends previous Burrows-Wheeler techniques… Continue reading
Cluster analysis and display of genome-wide expression patterns A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression. The output is displayed graphically, conveying the clustering and the underlying expression data simultaneously in a form intuitive… Continue reading
Biosynthesis of gold nanoparticles by foliar broths: roles of biocompounds and other attributes of the extracts. Biosynthesis of nanoparticles has arisen as a promising alternative to conventional synthetic methodologies owing to its eco-friendly advantages, and the involved bioprotocol still needs further clarification. This research, for the first time from the standpoint of statistics, confirmed an electrostatic force… Continue reading
Chromatin modifications and their function The surface of nucleosomes is studded with a multiplicity of modifications. At least eight different classes have been characterized to date and many different sites have been identified for each class. Operationally, modifications function either by disrupting chromatin contacts or by affecting the recruitment of nonhistone proteins to chromatin. Their… Continue reading
The fact is that siRNAs—the double-stranded effectors of RNA interference (RNAi)—can “be developed to silence any gene; can be developed incredibly fast; can have picomolar efficacy; [and] can have an effect that lasts for weeks.” In the lab, they have become invaluable tools, enabling the easy genetic knockdown of any sequence. The problem with migrating… Continue reading