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Ultrafast and memory-efficient alignment of short DNA sequences to the human genome

Posted on May 25, 2021April 29, 2021 by Oscar

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome Bowtie is an ultrafast, memory-efficient alignment program for aligning short DNA sequence reads to large genomes. For the human genome, Burrows-Wheeler indexing allows Bowtie to align more than 25 million reads per CPU hour with a memory footprint of approximately 1.3 gigabytes. Bowtie extends previous Burrows-Wheeler techniques… Continue reading

bioprotocols

Cluster analysis and display of genome-wide expression patterns

Posted on May 21, 2021April 27, 2021 by Oscar

Cluster analysis and display of genome-wide expression patterns A system of cluster analysis for genome-wide expression data from DNA microarray hybridization is described that uses standard statistical algorithms to arrange genes according to similarity in pattern of gene expression. The output is displayed graphically, conveying the clustering and the underlying expression data simultaneously in a form intuitive… Continue reading

Biosynthesis of g Hardware Design and Fault-Tolerant Synthesis for Digital Acoustofluidic Biochipsold nanoparticles by foliar broths: roles of biocompounds and other attributes of the extracts.

Posted on April 29, 2021April 29, 2021 by Oscar

Biosynthesis of gold nanoparticles by foliar broths: roles of biocompounds and other attributes of the extracts. Biosynthesis of nanoparticles has arisen as a promising alternative to conventional synthetic methodologies owing to its eco-friendly advantages, and the involved bioprotocol still needs further clarification. This research, for the first time from the standpoint of statistics, confirmed an electrostatic force… Continue reading

bioprotocols

Chromatin modifications and their function

Posted on April 27, 2021April 27, 2021 by Oscar

Chromatin modifications and their function The surface of nucleosomes is studded with a multiplicity of modifications. At least eight different classes have been characterized to date and many different sites have been identified for each class. Operationally, modifications function either by disrupting chromatin contacts or by affecting the recruitment of nonhistone proteins to chromatin. Their… Continue reading

Life Technologies

Posted on April 13, 2021April 13, 2021 by Oscar

The fact is that siRNAs—the double-stranded effectors of RNA interference (RNAi)—can “be developed to silence any gene; can be developed incredibly fast; can have picomolar efficacy; [and] can have an effect that lasts for weeks.” In the lab, they have become invaluable tools, enabling the easy genetic knockdown of any sequence. The problem with migrating… Continue reading

LB Medium and Agar Plate Recipe

Posted on April 13, 2021April 13, 2021 by Oscar

Protocol for LB liquid medium: For 1 liter, dissolve 10 g Tryptone, 5 g yeast extract, and 10 g NaCl in 950 ml deionized water. Adjust the pH of the solution to 7.0 with NaOH and bring the volume up to 1 liter.It may not be necessary to adjust pH every time, since pH of LB… Continue reading

Material safety data

Posted on April 13, 2021April 13, 2021 by Oscar

Use this calculator to prepare your solutions from solid reagents. Volume:  L Molarity:  M Molecular Weight:  gram/mol   Hit the submit button to calculate the weight of the reagent needed for the given volume to obtain desired molarity. Your employees should not have to guess whether or not they’re pulling up the correct SDS for… Continue reading

Molarity Calculator II

Posted on April 13, 2021 by Oscar

Use this calculator to adjust the concentration of a substance in a present solution to a higher concentration by adding a minimum volume of solution from a concentrated stock. Concentration of present solution:  mM Volume of present solution:  ml Stock Concentration:  mM Desired Final Concentration:  mM Hit the submit button to calculate the volume of… Continue reading

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